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Cellosaurus EJ-30 clone B3-WrnDN1 (CVCL_LN37)

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Cell line name EJ-30 clone B3-WrnDN1
Synonyms B3-WrnDN1
Accession CVCL_LN37
Resource Identification Initiative To cite this cell line use: EJ-30 clone B3-WrnDN1 (RRID:CVCL_LN37)
Comments Problematic cell line: Contaminated. Ancestral cell line (Ej138) has been shown to be a T24 derivative.
Population: Caucasian; Swedish.
Characteristics: Expresses WRN p.Lys577Met under the control of a tetracycline-inducible promoter.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:12791; WRN (Note=With p.Lys577Met).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.
Sequence variations
  • Mutation; HGNC; HGNC:5173; HRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012600; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr126Ter (c.378C>G); ClinVar=VCV000578988; Zygosity=Homozygous (from parent cell line).
Disease Bladder carcinoma (NCIt: C4912)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_LN36 (EJ-30 clone B3)
Sex of cell Female
Age at sampling 82Y
Category Cancer cell line
Publications

PubMed=12827497; DOI=10.1007/s00439-003-0972-y
Yun Bai, John Patrick Murnane;
Telomere instability in a human tumor cell line expressing a dominant-negative WRN protein.
Hum. Genet. 113:337-347(2003)

Cross-references
Cell line databases/resources cancercelllines; CVCL_LN37
Encyclopedic resources Wikidata; Q54832173
Entry history
Entry creation15-Nov-2017
Last entry update14-Aug-2025
Version number15