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Cellosaurus 3AB-OS (CVCL_LM95)

[Text version]
Cell line name 3AB-OS
Accession CVCL_LM95
Resource Identification Initiative To cite this cell line use: 3AB-OS (RRID:CVCL_LM95)
Comments Problematic cell line: Contaminated. Shown to be a RD derivative (personal communication of Millipore). Originally thought to be a MG-63 derivative.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00545.
Population: Caucasian.
Characteristics: Was thought to have cancer stem cell-like characteristics as it was thought to be produced by long term treatment of MG-63 with 3-aminobenzamide (3AB), an inhibitor of poly(ADP-ribose) polymerase.
Doubling time: ~22 hours (PubMed=19160414).
Selected for resistance to: ChEBI; CHEBI:64042; 3-aminobenzamide (3AB).
Omics: Array-based CGH.
Omics: CNV analysis.
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Pelvis, muscle; UBERON=UBERON_0001325.
Sequence variations
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61His (c.183A>T); ClinVar=VCV000373003; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg248Trp (c.742C>T); ClinVar=VCV000012347; Zygosity=Homozygous (from parent cell line).
Disease Embryonal rhabdomyosarcoma (NCIt: C8971)
Embryonal rhabdomyosarcoma (ORDO: Orphanet_99757)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1649 (RD)
Sex of cell Female
Age at sampling 7Y
Category Cancer cell line
STR profile Source(s): Millipore=SCC141

Penta D11,13
Penta E12

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx

PubMed=19160414; DOI=10.1002/jcp.21667
Di Fiore R., Santulli A., Drago-Ferrante R., Giuliano M., De Blasio A., Messina C., Pirozzi G., Tirino V., Tesoriere G., Vento R.
Identification and expansion of human osteosarcoma-cancer-stem cells by long-term 3-aminobenzamide treatment.
J. Cell. Physiol. 219:301-313(2009)

Di Fiore R., Drago-Ferrante R., D'Anneo A., De Blasio A., Santulli A., Messina C., Carlisi D., Tesoriere G., Vento R.
Differentiation of human osteosarcoma 3AB-OS stem-like cells in derivatives of the three primary germ layers as a useful in vitro model to develop several purposes.
Stem Cell Discov. 3:188-201(2013)

PubMed=23129384; DOI=10.1002/jcp.24272
Di Fiore R., Fanale D., Drago-Ferrante R., Chiaradonna F., Giuliano M., De Blasio A., Amodeo V., Corsini L.R., Bazan V., Tesoriere G., Vento R., Russo A.
Genetic and molecular characterization of the human osteosarcoma 3AB-OS cancer stem cell line: a possible model for studying osteosarcoma origin and stemness.
J. Cell. Physiol. 228:1189-1201(2013)

Di Fiore R., Drago-Ferrante R., Marcatti M., Carlisi D., Tesoriere G., Vento R.
A short story of 3AB-OS cancer stem cells, a possible model for studying cancer stemness.
Cancer Cell Microenviron. 1:e134.1-e134.5(2014)

Cell line collections (Providers) Millipore; SCC141 - Discontinued
Cell line databases/resources cancercelllines; CVCL_LM95
Encyclopedic resources Wikidata; Q54585282
Entry history
Entry creation15-Nov-2017
Last entry update02-May-2024
Version number15