ID   3AB-OS
AC   CVCL_LM95
DR   cancercelllines; CVCL_LM95
DR   Millipore; SCC141
DR   Wikidata; Q54585282
RX   DOI=10.14800/ccm.134;
RX   DOI=10.4236/scd.2013.33024;
RX   PubMed=19160414;
RX   PubMed=23129384;
WW   Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
CC   Problematic cell line: Contaminated. Shown to be a RD derivative (personal communication of Millipore). Originally thought to be a MG-63 derivative.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00545.
CC   Population: Caucasian.
CC   Characteristics: Was thought to have cancer stem cell-like characteristics as it was thought to be produced by long term treatment of MG-63 with 3-aminobenzamide (3AB), an inhibitor of poly(ADP-ribose) polymerase.
CC   Doubling time: ~22 hours (PubMed=19160414).
CC   Selected for resistance to: ChEBI; CHEBI_64042; 3-aminobenzamide (3AB).
CC   Sequence variation: Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61His (c.183A>T); ClinVar=VCV000373003; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg248Trp (c.742C>T); ClinVar=VCV000012347; Zygosity=Homozygous (from parent cell line).
CC   Omics: Transcriptomics; Microarray.
CC   Omics: Variations; Array-based CGH.
CC   Omics: Variations; CNV analysis.
CC   Discontinued: Millipore; SCC141; true.
CC   Derived from site: In situ; Pelvis, muscle; UBERON=UBERON_0001325.
ST   Source(s): Millipore=SCC141
ST   Amelogenin: X
ST   CSF1PO: 10,11
ST   D13S317: 13
ST   D16S539: 10,11
ST   D18S51: 13,18
ST   D21S11: 28,29
ST   D3S1358: 15,17
ST   D5S818: 11
ST   D7S820: 8,12
ST   D8S1179: 11,14,15
ST   FGA: 20,21
ST   Penta D: 11,13
ST   Penta E: 12
ST   TH01: 9.3
ST   TPOX: 9
ST   vWA: 18
DI   NCIt; C8971; Embryonal rhabdomyosarcoma
DI   ORDO; Orphanet_99757; Embryonal rhabdomyosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_1649 ! RD
SX   Female
AG   7Y
CA   Cancer cell line
DT   Created: 15-11-17; Last updated: 10-04-25; Version: 17
//
RX   PubMed=23129384; DOI=10.1002/jcp.24272;
RA   Di Fiore, Riccardo
RA   Fanale, Daniele
RA   Drago-Ferrante, Rosa
RA   Chiaradonna, Ferdinando
RA   Giuliano, Michela
RA   De Blasio, Anna
RA   Amodeo, Valeria
RA   Corsini, Lidia Rita
RA   Bazan, Viviana
RA   Tesoriere, Giovanni
RA   Vento, Renza
RA   Russo, Antonio
RT   "Genetic and molecular characterization of the human osteosarcoma
RT   3AB-OS cancer stem cell line: a possible model for studying
RT   osteosarcoma origin and stemness.";
RL   J. Cell. Physiol. 228:1189-1201(2013).
//
RX   DOI=10.4236/scd.2013.33024;
RA   Di Fiore, Riccardo
RA   Drago-Ferrante, Rosa
RA   D'Anneo, Antonella
RA   De Blasio, Anna
RA   Santulli, Andrea
RA   Messina, Concetta
RA   Carlisi, Daniela
RA   Tesoriere, Giovanni
RA   Vento, Renza
RT   "Differentiation of human osteosarcoma 3AB-OS stem-like cells in
RT   derivatives of the three primary germ layers as a useful in vitro
RT   model to develop several purposes.";
RL   Stem Cell Discov. 3:188-201(2013).
//
RX   PubMed=19160414; DOI=10.1002/jcp.21667;
RA   Di Fiore, Riccardo
RA   Santulli, Andrea
RA   Drago-Ferrante, Rosa
RA   Giuliano, Michela
RA   De Blasio, Anna
RA   Messina, Concetta
RA   Pirozzi, Giuseppe
RA   Tirino, Virginia
RA   Tesoriere, Giovanni
RA   Vento, Renza
RT   "Identification and expansion of human osteosarcoma-cancer-stem cells
RT   by long-term 3-aminobenzamide treatment.";
RL   J. Cell. Physiol. 219:301-313(2009).
//
RX   DOI=10.14800/ccm.134;
RA   Di Fiore, Riccardo
RA   Drago-Ferrante, Rosa
RA   Marcatti, Michela
RA   Carlisi, Daniela
RA   Tesoriere, Giovanni
RA   Vento, Renza
RT   "A short story of 3AB-OS cancer stem cells, a possible model for
RT   studying cancer stemness.";
RL   Cancer Cell Microenviron. 1:e134.1-e134.5(2014).
//