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Cellosaurus tsET24 (CVCL_LJ52)

[Text version]
Cell line name tsET24
Synonyms ts ET24; ET24
Accession CVCL_LJ52
Resource Identification Initiative To cite this cell line use: tsET24 (RRID:CVCL_LJ52)
Comments Characteristics: Temperature sensitive. At the non-permissive temperature of 39.5 Celsius, this cell line is arrested in the G1-phase and survives for more than 3 days.
Transformant: ChEBI; CHEBI:21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
Derived from sampling site: Kidney.
Sequence variations
  • Mutation; UniProtKB; Q8K5D5; Ddx3x; Simple; p.Pro267Ser (c.799C>T); Zygosity=Unspecified (PubMed=12944373).
Species of origin Mesocricetus auratus (Golden hamster) (NCBI Taxonomy: 10036)
Hierarchy Parent: CVCL_1915 (BHK-21 clone 13)
Sex of cell Male
Age at sampling 1D
Category Spontaneously immortalized cell line

PubMed=9251028; DOI=10.1016/S0076-6879(97)83024-8
Nishimoto T.
Isolation and characterization of temperature-sensitive mammalian cell cycle mutants.
Methods Enzymol. 283:292-309(1997)

PubMed=12944373; DOI=10.1093/jb/mvg126
Fukumura J., Noguchi E., Sekiguchi T., Nishimoto T.
A temperature-sensitive mutant of the mammalian RNA helicase, DEAD-BOX X isoform, DBX, defective in the transition from G1 to S phase.
J. Biochem. 134:71-82(2003)

PubMed=15383328; DOI=10.1016/j.yexcr.2004.07.005
Sekiguchi T., Iida H., Fukumura J., Nishimoto T.
Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation.
Exp. Cell Res. 300:213-222(2004)

Encyclopedic resources Wikidata; Q54973119
Entry history
Entry creation15-Nov-2017
Last entry update20-May-2021
Version number5