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Cellosaurus CHO-K1 tsTM3 (CVCL_LH72)

[Text version]
Cell line name CHO-K1 tsTM3
Synonyms tsTM3
Accession CVCL_LH72
Resource Identification Initiative To cite this cell line use: CHO-K1 tsTM3 (RRID:CVCL_LH72)
Comments Characteristics: Temperature sensitive. Exhibits chromosomal instability and cell-cycle arrest at S and G2 phases with decreased DNA synthesis at the non-permissive temperature of 39 Celsius.
Transformant: ChEBI; CHEBI_21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Mutation; UniProtKB; G3IBG3; Uba1; Simple; p.Met256Ile (c.768G>A); Zygosity=Unspecified (PubMed=24805847).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=2237640; DOI=10.1007/BF01233196
Hideo Tsuji, Yasushi Matsudo, Satsuki Tsuji, Fumio Hanaoka, Masao Hyodo, Tada-aki Hori;
Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting chromosomal instability and reduced DNA synthesis at nonpermissive temperature.
Somat. Cell Mol. Genet. 16:461-476(1990)

PubMed=24805847; DOI=10.1371/journal.pone.0096666; PMCID=PMC4013028
Kimihiko Sugaya, Yoshie Ishihara, Sonoe Inoue, Hideo Tsuji;
Characterization of ubiquitin-activating enzyme Uba1 in the nucleus by its mammalian temperature-sensitive mutant.
PLoS ONE 9:e96666.1-e96666.12(2014)

PubMed=26059705; DOI=10.1111/gtc.12257
Kimihiko Sugaya, Yoshie Ishihara, Sonoe Inoue;
Nuclear localization of ubiquitin-activating enzyme Uba1 is characterized in its mammalian temperature-sensitive mutant.
Genes Cells 20:659-666(2015)

Cross-references
Encyclopedic resources Wikidata; Q54973165
Entry history
Entry creation15-Nov-2017
Last entry update19-Dec-2024
Version number9