ID   CHO-K1 tsTM3
AC   CVCL_LH72
SY   tsTM3
DR   Wikidata; Q54973165
RX   PubMed=2237640;
RX   PubMed=24805847;
RX   PubMed=26059705;
CC   Characteristics: Temperature sensitive. Exhibits chromosomal instability and cell-cycle arrest at S and G2 phases with decreased DNA synthesis at the non-permissive temperature of 39 Celsius.
CC   Sequence variation: Mutation; UniProtKB; G3IBG3; Uba1; Simple; p.Met256Ile (c.768G>A); Zygosity=Unspecified (PubMed=24805847).
CC   Transformant: ChEBI; CHEBI:21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_0214 ! CHO-K1
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 15-11-17; Last updated: 30-01-24; Version: 8
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RX   PubMed=2237640; DOI=10.1007/BF01233196;
RA   Tsuji H., Matsudo Y., Tsuji S., Hanaoka F., Hyodo M., Hori T.-a.;
RT   "Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting
RT   chromosomal instability and reduced DNA synthesis at nonpermissive
RT   temperature.";
RL   Somat. Cell Mol. Genet. 16:461-476(1990).
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RX   PubMed=24805847; DOI=10.1371/journal.pone.0096666;
RA   Sugaya K., Ishihara Y., Inoue S., Tsuji H.;
RT   "Characterization of ubiquitin-activating enzyme Uba1 in the nucleus
RT   by its mammalian temperature-sensitive mutant.";
RL   PLoS ONE 9:E96666-E96666(2014).
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RX   PubMed=26059705; DOI=10.1111/gtc.12257;
RA   Sugaya K., Ishihara Y., Inoue S.;
RT   "Nuclear localization of ubiquitin-activating enzyme Uba1 is
RT   characterized in its mammalian temperature-sensitive mutant.";
RL   Genes Cells 20:659-666(2015).
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