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Cellosaurus ts422E (CVCL_LH71)

[Text version]
Cell line name ts422E
Synonyms ts 422E; ts-422E; 422E
Accession CVCL_LH71
Resource Identification Initiative To cite this cell line use: ts422E (RRID:CVCL_LH71)
Comments Characteristics: Temperature sensitive. Loses the ability to synthesize polyamines and 5.8s rRNA at the non-permissive temperature (39 Celsius).
Doubling time: 16 hours, at 33 Celsius (PubMed=4515626).
Transformant: ChEBI; CHEBI:60761; 5-fluoro-2'-deoxyuridine (FUdR).
Derived from sampling site: Kidney.
Species of origin Mesocricetus auratus (Golden hamster) (NCBI Taxonomy: 10036)
Hierarchy Parent: CVCL_1915 (BHK-21 clone 13)
Sex of cell Male
Age at sampling 1D
Category Transformed cell line

PubMed=4267115; DOI=10.1038/newbio239066a0
Meiss H.K., Basilico C.
Temperature sensitive mutants of BHK 21 cells.
Nature New Biol. 239:66-68(1972)

PubMed=4515626; DOI=10.1073/pnas.70.4.1273
Toniolo D., Meiss H.K., Basilico C.
A temperature-sensitive mutation affecting 28S ribosomal RNA production in mammalian cells.
Proc. Natl. Acad. Sci. U.S.A. 70:1273-1277(1973)

PubMed=291978; DOI=10.1073/pnas.76.9.4469
Kumar A.
Regulation of translational initiation in a temperature-sensitive mutant of BHK cells.
Proc. Natl. Acad. Sci. U.S.A. 76:4469-4473(1979)

PubMed=528570; DOI=10.1002/jcp.1041010303
Levin E.G., Clark J.L.
Defect in polyamine metabolism in a BHK cell mutant temperature-sensitive for rRNA maturation.
J. Cell. Physiol. 101:361-368(1979)

Encyclopedic resources Wikidata; Q54973093
Entry history
Entry creation15-Nov-2017
Last entry update02-Jul-2020
Version number4