ID   ts422E
AC   CVCL_LH71
SY   ts 422E; ts-422E; 422E
DR   Wikidata; Q54973093
RX   PubMed=291978;
RX   PubMed=528570;
RX   PubMed=4267115;
RX   PubMed=4515626;
CC   Characteristics: Temperature sensitive. Loses the ability to synthesize polyamines and 5.8s rRNA at the non-permissive temperature (39 Celsius).
CC   Doubling time: 16 hours (Note=At 33 Celsius) (PubMed=4515626).
CC   Transformant: ChEBI; CHEBI_60761; 5-fluoro-2'-deoxyuridine (FUdR).
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=10036; ! Mesocricetus auratus (Golden hamster)
HI   CVCL_1915 ! BHK-21 clone 13
SX   Male
AG   1D
CA   Transformed cell line
DT   Created: 15-11-17; Last updated: 19-12-24; Version: 7
//
RX   PubMed=291978; DOI=10.1073/pnas.76.9.4469; PMCID=PMC411598;
RA   Kumar A.;
RT   "Regulation of translational initiation in a temperature-sensitive
RT   mutant of BHK cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:4469-4473(1979).
//
RX   PubMed=528570; DOI=10.1002/jcp.1041010303;
RA   Levin E.G., Clark J.L.;
RT   "Defect in polyamine metabolism in a BHK cell mutant
RT   temperature-sensitive for rRNA maturation.";
RL   J. Cell. Physiol. 101:361-368(1979).
//
RX   PubMed=4267115; DOI=10.1038/newbio239066a0;
RA   Meiss H.K., Basilico C.;
RT   "Temperature sensitive mutants of BHK 21 cells.";
RL   Nat. New Biol. 239:66-68(1972).
//
RX   PubMed=4515626; DOI=10.1073/pnas.70.4.1273; PMCID=PMC433474;
RA   Toniolo D., Meiss H.K., Basilico C.;
RT   "A temperature-sensitive mutation affecting 28S ribosomal RNA
RT   production in mammalian cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 70:1273-1277(1973).
//