Cellosaurus cell line tsAF8 (CVCL

Cellosaurus tsAF8 (CVCL_LH56)

Cross-references
Cell line name	tsAF8
Synonyms	ts AF8; AF8
Accession	CVCL_LH56
Resource Identification Initiative	To cite this cell line use: tsAF8 (RRID:CVCL_LH56)
Comments	Characteristics: Temperature sensitive. Arrests at the non-permissive temperature (40.6 Celsius) in the mid-G1 phase and is deficient in RNA polymerase II activity. Doubling time: ~18 hours (Note=At 33 Celsius) (PubMed=4373487). Transformant: ChEBI; CHEBI_60761; 5-fluoro-2'-deoxyuridine (FUdR). Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Sequence variations	Mutation; UniProtKB; A0A1U8BM24; Polr2a; Simple; p.Ala315Asp (c.944C>A); Zygosity=Heterozygous (PubMed=11674999).
Species of origin	Mesocricetus auratus (Golden hamster) (NCBI Taxonomy: 10036)
Hierarchy	Parent: CVCL_1915 (BHK-21 clone 13)
Sex of cell	Male
Age at sampling	1D
Category	Transformed cell line
Publications	PubMed=4267115; DOI=10.1038/newbio239066a0 Harriet Kaufmann Meiss, Claudio Basilico; Temperature sensitive mutants of BHK 21 cells. Nat. New Biol. 239:66-68(1972) PubMed=4373487; DOI=10.1002/jcp.1040840308 Stuart Jay Burstin, Harriet Kaufmann Meiss, Claudio Basilico; A temperature-sensitive cell cycle mutant of the BHK cell line. J. Cell. Physiol. 84:397-408(1974) PubMed=7191857; DOI=10.1002/jcp.1041030114 Mara Rossini, Susan Baserga, Constance H. Huang, C. James Ingles, Renato Baserga; Changes in RNA polymerase II in a cell cycle-specific temperature-sensitive mutant of hamster cells. J. Cell. Physiol. 103:97-103(1980) PubMed=11674999; DOI=10.1016/S0378-1119(01)00615-1 Kimihiko Sugaya, Shun-ichi Sasanuma, Peter R. Cook, Kazuei Mita; A mutation in the largest (catalytic) subunit of RNA polymerase II and its relation to the arrest of the cell cycle in G(1) phase. Gene 274:77-81(2001)
Encyclopedic resources	Wikidata; Q54973101
Entry history
Entry creation	15-Nov-2017
Last entry update	19-Dec-2024
Version number	9