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Cellosaurus tsAF8 (CVCL_LH56)

[Text version]
Cell line name tsAF8
Synonyms ts AF8; AF8
Accession CVCL_LH56
Resource Identification Initiative To cite this cell line use: tsAF8 (RRID:CVCL_LH56)
Comments Characteristics: Temperature sensitive. Arrests at the non-permissive temperature (40.6 Celsius) in the mid-G1 phase and is deficient in RNA polymerase II activity.
Doubling time: ~18 hours, at 33 Celsius (PubMed=4373487).
Transformant: ChEBI; CHEBI:60761; 5-fluoro-2'-deoxyuridine (FUdR).
Derived from sampling site: Kidney.
Sequence variations
  • Mutation; UniProtKB; A0A1U8BM24; Polr2a; Simple; p.Ala315Asp (c.944C>A); Zygosity=Heterozygous (PubMed=11674999).
Species of origin Mesocricetus auratus (Golden hamster) (NCBI Taxonomy: 10036)
Hierarchy Parent: CVCL_1915 (BHK-21 clone 13)
Sex of cell Male
Age at sampling 1D
Category Transformed cell line

PubMed=4267115; DOI=10.1038/newbio239066a0
Meiss H.K., Basilico C.
Temperature sensitive mutants of BHK 21 cells.
Nature New Biol. 239:66-68(1972)

PubMed=4373487; DOI=10.1002/jcp.1040840308
Burstin S.J., Meiss H.K., Basilico C.
A temperature-sensitive cell cycle mutant of the BHK cell line.
J. Cell. Physiol. 84:397-408(1974)

PubMed=7191857; DOI=10.1002/jcp.1041030114
Rossini M., Baserga S., Huang C.H., Ingles C.J., Baserga R.
Changes in RNA polymerase II in a cell cycle-specific temperature-sensitive mutant of hamster cells.
J. Cell. Physiol. 103:97-103(1980)

PubMed=11674999; DOI=10.1016/S0378-1119(01)00615-1
Sugaya K., Sasanuma S.-I., Cook P.R., Mita K.
A mutation in the largest (catalytic) subunit of RNA polymerase II and its relation to the arrest of the cell cycle in G(1) phase.
Gene 274:77-81(2001)

Encyclopedic resources Wikidata; Q54973101
Entry history
Entry creation15-Nov-2017
Last entry update23-Jun-2022
Version number6