ID   tsAF8
AC   CVCL_LH56
SY   ts AF8; AF8
DR   Wikidata; Q54973101
RX   PubMed=4267115;
RX   PubMed=4373487;
RX   PubMed=7191857;
RX   PubMed=11674999;
CC   Characteristics: Temperature sensitive. Arrests at the non-permissive temperature (40.6 Celsius) in the mid-G1 phase and is deficient in RNA polymerase II activity.
CC   Doubling time: ~18 hours (Note=At 33 Celsius) (PubMed=4373487).
CC   Sequence variation: Mutation; UniProtKB; A0A1U8BM24; Polr2a; Simple; p.Ala315Asp (c.944C>A); Zygosity=Heterozygous (PubMed=11674999).
CC   Transformant: ChEBI; CHEBI:60761; 5-fluoro-2'-deoxyuridine (FUdR).
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=10036; ! Mesocricetus auratus (Golden hamster)
HI   CVCL_1915 ! BHK-21 clone 13
SX   Male
AG   1D
CA   Transformed cell line
DT   Created: 15-11-17; Last updated: 29-06-23; Version: 8
//
RX   PubMed=4267115; DOI=10.1038/newbio239066a0;
RA   Meiss H.K., Basilico C.;
RT   "Temperature sensitive mutants of BHK 21 cells.";
RL   Nature New Biol. 239:66-68(1972).
//
RX   PubMed=4373487; DOI=10.1002/jcp.1040840308;
RA   Burstin S.J., Meiss H.K., Basilico C.;
RT   "A temperature-sensitive cell cycle mutant of the BHK cell line.";
RL   J. Cell. Physiol. 84:397-408(1974).
//
RX   PubMed=7191857; DOI=10.1002/jcp.1041030114;
RA   Rossini M., Baserga S., Huang C.H., Ingles C.J., Baserga R.;
RT   "Changes in RNA polymerase II in a cell cycle-specific
RT   temperature-sensitive mutant of hamster cells.";
RL   J. Cell. Physiol. 103:97-103(1980).
//
RX   PubMed=11674999; DOI=10.1016/S0378-1119(01)00615-1;
RA   Sugaya K., Sasanuma S.-i., Cook P.R., Mita K.;
RT   "A mutation in the largest (catalytic) subunit of RNA polymerase II
RT   and its relation to the arrest of the cell cycle in G(1) phase.";
RL   Gene 274:77-81(2001).
//