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Cellosaurus NISK9 (CVCL_LB87)

[Text version]
Cell line name NISK9
Synonyms NISK 9
Accession CVCL_LB87
Resource Identification Initiative To cite this cell line use: NISK9 (RRID:CVCL_LB87)
Comments Group: Patented cell line.
Registration: International Depositary Authority, European Collection of Cell Cultures (ECACC); 09709106.
Characteristics: Insulin secreting.
Characteristics: Compared to the parent cell line, it has a repaired ATP-regulated potassium channel activity.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:59; ABCC8.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:6257; KCNJ11.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:6107; PDX1.
Disease Persistent hyperinsulinemic hypoglycemia of infancy (NCIt: C122923)
Autosomal dominant hyperinsulinism due to SUR1 deficiency (ORDO: Orphanet_276575)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_LB86 (NES2Y)
Category Spontaneously immortalized cell line
Publications

PubMed=10567373; DOI=10.1074/jbc.274.48.34059
Macfarlane W.M., Chapman J.C., Shepherd R.M., Hashmi M.N., Kamimura N., Cosgrove K.E., O'Brien R.E., Barnes P.D., Hart A.W., Docherty H.M., Lindley K.J., Aynsley-Green A., James R.F.L., Docherty K., Dunne M.J.
Engineering a glucose-responsive human insulin-secreting cell line from islets of Langerhans isolated from a patient with persistent hyperinsulinemic hypoglycemia of infancy.
J. Biol. Chem. 274:34059-34066(1999)

Patent=US6372501
Aynsley-Green A., Lindley K.J., Docherty K., Dunne M.J., Macfarlane W.M., James R.F.L.
Glucose responsive beta-cell line.
Patent number US6372501, 16-Apr-2002

Cross-references
Encyclopedic resources Wikidata; Q54930632
Entry history
Entry creation22-Aug-2017
Last entry update19-Dec-2024
Version number8