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Cellosaurus CMT12 (CVCL_L329)

[Text version]
Cell line name CMT12
Synonyms CMT-12; Canine Mammary Tumor 12; CMT-2; CMT2; Canine Mammary Tumor-2
Accession CVCL_L329
Secondary accession CVCL_X219
Resource Identification Initiative To cite this cell line use: CMT12 (RRID:CVCL_L329)
Comments Part of: FACC canine tumor cell line panel.
Doubling time: ~24 hours (PubMed=3462415); 19 hours (PubMed=27257868).
Omics: Genomics; Whole exome sequencing.
Omics: Transcriptomics; Microarray.
Caution: CMT-2 was renamed CMT-12 (PubMed=11848473).
Caution: On the basis of the STR profile CMT12 and CMT27 seem to originate from the same donor (PubMed=21908323; PubMed=27197945).
Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
Sequence variations
  • Mutation; VGNC; VGNC:42490; KMT2C; Simple; p.Arg1201fs (c.3600delGinsAA); Zygosity=Homozygous (PubMed=31175136).
  • Mutation; VGNC; VGNC:47719; TP53; Simple; p.Val132Glu (c.395T>A); Zygosity=Homozygous (PubMed=31175136).
Disease Canine mammary carcinoma (NCIt: C124249)
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Poodle; VBO=VBO_0201048.
Hierarchy Children:
CVCL_C1IG (CMT-12CnB7-1R25)CVCL_C1IH (CMT-12CnB7-1R26)CVCL_C1II (CMT-12CnB7-2Q2)
CVCL_C1IJ (CMT-12HuB7-2C10)CVCL_C1H3 (CMT-2 c1)CVCL_C1H4 (CMT-2 c2)
CVCL_C1H5 (CMT-2 c3)CVCL_C1H6 (CMT-2 c4)CVCL_C1H7 (CMT-2 c5)
Originate from same individual CVCL_1R41 ! CMT27
Sex of cell Female
Age at sampling >10Y
Category Cancer cell line
STR profile Source(s): PubMed=27197945; PubMed=31175136

Markers:
Dog FHC2010235
Dog FHC2054151,176
Dog FHC2079271
Dog PEZ1115
Dog PEZ12277
Dog PEZ20176
Dog PEZ3113,119
Dog PEZ5103
Dog PEZ6176
Dog PEZ8233,244

Run an STR similarity search on this cell line
Publications

PubMed=3462415; DOI=10.1093/jnci/77.3.783
Lauren G. Wolfe, Bonnie Buxton Smith, Maria A. Toivio-Kinnucan, Eva Ann Sartin, Robert Paul Kwapien, Ralph A. Henderson, Stephen Barnes;
Biologic properties of cell lines derived from canine mammary carcinomas.
J. Natl. Cancer Inst. 77:783-792(1986)

CLPUB00711
Eva Ann Sartin;
Biological properties of canine mammary tumors and cell lines.
Thesis PhD (1990); Auburn University College of Veterinary Medicine; Auburn; USA

PubMed=8476218
Eva Ann Sartin, Stephen Barnes, Maria A. Toivio-Kinnucan, James C. Wright, Lauren G. Wolfe;
Heterogenic properties of clonal cell lines derived from canine mammary carcinomas and sensitivity to tamoxifen and doxorubicin.
Anticancer Res. 13:229-236(1993)

PubMed=11848473
Vendil Vatne, Jorunn Litlekalsoey, Frits Thorsen, Gro O. Ness, Tore Wentzel-Larsen, Jens Gerhard Hostmark;
Characterization of cell lines originating from an invasive transitional cell carcinoma.
Anticancer Res. 21:3199-3207(2001)

PubMed=12529965
Elizabeth M. Whitley, Karen S. Sellins, Sandra J. Ewald, Bruce F. Smith, Shu-Min Yang, Eva Ann Sartin, Lauren G. Wolfe;
Canine mammary tumor cells transfected with B7-1 or B7-2 stimulate proliferation of peripheral blood mononuclear cells.
Anticancer Res. 22:2567-2574(2002)

PubMed=19130492; DOI=10.1002/jcb.22034
Patricia DeInnocentes, Payal Agarwal, R. Curtis Bird;
Phenotype-rescue of cyclin-dependent kinase inhibitor p16/INK4A defects in a spontaneous canine cell model of breast cancer.
J. Cell. Biochem. 106:491-505(2009)

PubMed=21801068; DOI=10.2460/ajvr.72.8.1097
Tracy Stokol, Janelle L. Daddona, Lamya S. Mubayed, Jakob Trimpert, Sungkwon Kang;
Evaluation of tissue factor expression in canine tumor cells.
Am. J. Vet. Res. 72:1097-1106(2011)

PubMed=21908323; DOI=10.1177/1040638711408064
Liza E. O'Donoghue, Jason P. Rivest, Dawn L. Duval;
Polymerase chain reaction-based species verification and microsatellite analysis for canine cell line validation.
J. Vet. Diagn. Invest. 23:780-785(2011)

DOI=10.4236/ojvm.2012.24045
Cheryl E. Balkman, Tracy L. Gieger, Marsha M. Zgola, Lionel D. Lewis, Margaret C. McEntee;
In vitro characterization of docetaxel as a radiosensitizer in canine and feline cancer cell lines.
Open J. Vet. Med. 2:285-292(2012)

PubMed=23238983; DOI=10.1002/jcb.24476
Payal Agarwal, Maninder Sandey, Patricia DeInnocentes, R. Curtis Bird;
Tumor suppressor gene p16/INK4A/CDKN2A-dependent regulation into and out of the cell cycle in a spontaneous canine model of breast cancer.
J. Cell. Biochem. 114:1355-1363(2013)

PubMed=27257868; DOI=10.1371/journal.pone.0156689; PMCID=PMC4892608
Junko Maeda, Coral Eva Froning, Colleen A. Brents, Barbara J. Rose, Douglas H. Thamm, Takamitsu A. Kato;
Intrinsic radiosensitivity and cellular characterization of 27 canine cancer cell lines.
PLoS ONE 11:e0156689.1-e0156689.18(2016)

PubMed=27197945; DOI=10.1111/vco.12192; PMCID=PMC5656225
Jared S. Fowles, Deanna D. Dailey, Daniel L. Gustafson, Douglas H. Thamm, Dawn L. Duval;
The Flint Animal Cancer Center (FACC) canine tumour cell line panel: a resource for veterinary drug discovery, comparative oncology and translational medicine.
Vet. Comp. Oncol. 15:481-492(2017)

PubMed=31175136; DOI=10.1158/1535-7163.MCT-18-1346; PMCID=PMC6679748
Sunetra Das, Rupa Idate, Kathryn E. Cronise, Daniel L. Gustafson, Dawn L. Duval;
Identifying candidate druggable targets in canine cancer cell lines using whole-exome sequencing.
Mol. Cancer Ther. 18:1460-1471(2019)

Cross-references
Encyclopedic resources Wikidata; Q54813839
Gene expression databases GEO; GSM1974783
Entry history
Entry creation06-May-2013
Last entry update10-Apr-2025
Version number22