Cellosaurus cell line HepG2 Tet-Off Advanced (CVCL

Cross-references
Cell line name	HepG2 Tet-Off Advanced
Accession	CVCL_KU36
Resource Identification Initiative	To cite this cell line use: HepG2 Tet-Off Advanced (RRID:CVCL_KU36)
Comments	Population: Caucasian. Characteristics: Transfected with an advanced tetracycline transactivator (tTA2(S)-M2) protein, a fusion of a mutated E.coli tetR with the activation domain of HSV-1 VP16. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del). Discontinued: Takara/Clontech; Catalog number 631151. Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations	Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease	Hepatoblastoma (NCIt: C3728) Hepatoblastoma (ORDO: Orphanet_449)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0027 (Hep-G2)
Sex of cell	Male
Age at sampling	15Y
Category	Cancer cell line
Cell line databases/resources	cancercelllines; CVCL_KU36
Encyclopedic resources	Wikidata; Q54882846
Entry history
Entry creation	22-Aug-2017
Last entry update	14-Aug-2025
Version number	18