Cellosaurus cell line DKFZ-BT66 (CVCL

Cellosaurus DKFZ-BT66 (CVCL_JX58)

Cross-references
Cell line name	DKFZ-BT66
Accession	CVCL_JX58
Resource Identification Initiative	To cite this cell line use: DKFZ-BT66 (RRID:CVCL_JX58)
Comments	Characteristics: Transduced with a doxycycline-inducible system coding for SV40 large T antigen and RFP (PubMed=28002790). Senescence: Withdrawal of doxycycline leads to senescence (PubMed=28002790). Doubling time: 45 hours (PubMed=28002790). Genetic integration: Method=Transduction; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.). Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Brain, cerebellum; UBERON=UBERON_0002037.
Sequence variations	Gene fusion; HGNC; HGNC:1097; BRAF + HGNC; HGNC:22219; KIAA1549; Name(s)=KIAA1549-BRAF; Note=KIAA1549 exon 16 fused to BRAF exon 9 (PubMed=28002790).
Disease	Pilocytic astrocytoma (NCIt: C4047) Pilocytic astrocytoma (ORDO: Orphanet_251612)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell	Male
Age at sampling	2Y
Category	Cancer cell line
Publications	PubMed=28002790; DOI=10.18632/oncotarget.14004; PMCID=PMC5355278 Florian Selt, Juliane Hohloch, Thomas Hielscher, Felix Sahm, David Capper, Andrey Korshunov, Diren Usta, Sebastian Brabetz, Johannes Ridinger, Jonas Ecker, Ina Oehme ...Show all 24 authors... , Jan Gronych, Viktoria Marquardt, David Pauck, Heidi Bachli, Charles Dean Stiles, Andreas von Deimling, Marc Remke, Martin U. Schuhmann, Stefan Michael Pfister, Tilman Brummer, David T.W. Jones, Olaf Witt, Till Milde; Show fewer authors Establishment and application of a novel patient-derived KIAA1549:BRAF-driven pediatric pilocytic astrocytoma model for preclinical drug testing. Oncotarget 8:11460-11479(2017) PubMed=35977048; DOI=10.1093/neuonc/noac199; PMCID=PMC10076946 Florian Selt, Romain Sigaud, Gintvile Valinciute, Philipp Sievers, Julia Zaman, Clara Alco, Simone Schmid, Heike Peterziel, Jessica W. Tsai, Romain Guiho, Juan Pedro Martinez-Barbera ...Show all 28 authors... , Stefan Pusch, Jing Deng, Yi-Fan Zhai, Cornelis M. van Tilburg, Martin U. Schuhmann, Ahmed El Damaty, Pratiti Bandopadhayay, Christel Herold-Mende, Andreas von Deimling, Stefan Michael Pfister, Joan Montero, David Capper, Ina Oehme, Felix Sahm, David T.W. Jones, Olaf Witt, Till Milde; Show fewer authors BH3 mimetics targeting BCL-XL impact the senescent compartment of pilocytic astrocytoma. Neuro-oncol. 25:735-747(2023)
Encyclopedic resources	Wikidata; Q54831117
Entry history
Entry creation	22-Aug-2017
Last entry update	14-Aug-2025
Version number	11