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Cellosaurus DKFZ-BT66 (CVCL_JX58)

[Text version]
Cell line name DKFZ-BT66
Accession CVCL_JX58
Resource Identification Initiative To cite this cell line use: DKFZ-BT66 (RRID:CVCL_JX58)
Comments Characteristics: Transduced with a doxycycline-inducible system coding for SV40 large T antigen and RFP (PubMed=28002790).
Senescence: Withdrawal of doxycycline leads to senescence (PubMed=28002790).
Doubling time: 45 hours (PubMed=28002790).
Genetic integration: Method=Transduction; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Brain, cerebellum; UBERON=UBERON_0002037.
Sequence variations
  • Gene fusion; HGNC; HGNC:1097; BRAF + HGNC; HGNC:22219; KIAA1549; Name(s)=KIAA1549-BRAF; Note=KIAA1549 exon 16 fused to BRAF exon 9 (PubMed=28002790).
Disease Pilocytic astrocytoma (NCIt: C4047)
Pilocytic astrocytoma (ORDO: Orphanet_251612)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 2Y
Category Cancer cell line
Publications

PubMed=28002790; DOI=10.18632/oncotarget.14004; PMCID=PMC5355278
Florian Selt, Juliane Hohloch, Thomas Hielscher, Felix Sahm, David Capper, Andrey Korshunov, Diren Usta, Sebastian Brabetz, Johannes Ridinger, Jonas Ecker, Ina Oehme ...Show all 24 authors... , Jan Gronych, Viktoria Marquardt, David Pauck, Heidi Bachli, Charles Dean Stiles, Andreas von Deimling, Marc Remke, Martin U. Schuhmann, Stefan Michael Pfister, Tilman Brummer, David T.W. Jones, Olaf Witt, Till Milde; Show fewer authors
Establishment and application of a novel patient-derived KIAA1549:BRAF-driven pediatric pilocytic astrocytoma model for preclinical drug testing.
Oncotarget 8:11460-11479(2017)

PubMed=35977048; DOI=10.1093/neuonc/noac199; PMCID=PMC10076946
Florian Selt, Romain Sigaud, Gintvile Valinciute, Philipp Sievers, Julia Zaman, Clara Alco, Simone Schmid, Heike Peterziel, Jessica W. Tsai, Romain Guiho, Juan Pedro Martinez-Barbera ...Show all 28 authors... , Stefan Pusch, Jing Deng, Yi-Fan Zhai, Cornelis M. van Tilburg, Martin U. Schuhmann, Ahmed El Damaty, Pratiti Bandopadhayay, Christel Herold-Mende, Andreas von Deimling, Stefan Michael Pfister, Joan Montero, David Capper, Ina Oehme, Felix Sahm, David T.W. Jones, Olaf Witt, Till Milde; Show fewer authors
BH3 mimetics targeting BCL-XL impact the senescent compartment of pilocytic astrocytoma.
Neuro-oncol. 25:735-747(2023)

Cross-references
Encyclopedic resources Wikidata; Q54831117
Entry history
Entry creation22-Aug-2017
Last entry update14-Aug-2025
Version number11