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Cellosaurus K-562-GFP (CVCL_JM00)

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Cell line name K-562-GFP
Accession CVCL_JM00
Resource Identification Initiative To cite this cell line use: K-562-GFP (RRID:CVCL_JM00)
Comments Population: Caucasian.
Characteristics: Stably expresses GFP under the control of the human EF1a promoter.
Doubling time: 18-24 hours (ATCC=CCL-243-GFP).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).
Disease Blast phase chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C9110)
Chronic myeloid leukemia (ORDO: Orphanet_521)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0004 (K-562)
Sex of cell Female
Age at sampling 53Y
Category Cancer cell line
STR profile Source(s): ATCC=CCL-243-GFP

Markers:
AmelogeninX
CSF1PO9,10
D5S81811,12
D7S8209,11
D13S3178
D16S53911,12
TH019.3
TPOX8,9
vWA16

Run an STR similarity search on this cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/population-doubling-time-of-k-562-gfp
Publications

CLPUB00577
Helen Penney McWilliams-Koeppen, James Clinton;
A simple and rapid alternative to 51chromium or fluorescent dye loading for quantification of natural killer cell activity: ATCC K-562-GFP cells.
(In technical document) ATCC application note 31; pp.1-8; ATCC; Manassas; USA (2017)

Cross-references
Cell line collections (Providers) ATCC; CCL-243-GFP
Encyclopedic resources Wikidata; Q54899220
Entry history
Entry creation15-May-2017
Last entry update14-Aug-2025
Version number21