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Cellosaurus A549 EML4-ALK (CVCL_JK07)

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Cell line name A549 EML4-ALK
Synonyms EML4-ALK Fusion-A549 Isogenic Cell Line
Accession CVCL_JK07
Resource Identification Initiative To cite this cell line use: A549 EML4-ALK (RRID:CVCL_JK07)
Comments Population: Caucasian.
Characteristics: An EML4-ALK4 fusion was generated by CRISPR/Cas9.
Characteristics: More sensitive to the ALK inhibitor crizotinib when compared to the parental cell line. Can be a useful model to study tyrosine kinase signaling pathway and to screen ALK inhibitors.
Doubling time: ~25 hours (ATCC=CCL-185IG).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0023 (A-549)
Children:
CVCL_VR66 (A549 EML4-ALK-Luc2)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): ATCC=CCL-185IG

Markers:
AmelogeninX
CSF1PO10,12
D5S81811
D7S8208,11
D13S31711
D16S53911,12
TH018,9.3
TPOX8,11
vWA14

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Publications

CLPUB00395
Metewo Selase Enuameh, Lysa-Anne Volpe, Michael Jackson, John Foulke, Lu-Ping Chen, Brian A. Shapiro, Fang Tian;
The generation of an EML4-ALK fusion NSCLC isogenic cell line relevant for drug discovery and development.
(In technical document) ATCC application note 25; pp.1-4; ATCC; Manassas; USA (2016)

Cross-references
Cell line collections (Providers) ATCC; CCL-185IG
Cell line databases/resources cancercelllines; CVCL_JK07
Encyclopedic resources Wikidata; Q54606944
Entry history
Entry creation15-May-2017
Last entry update19-Dec-2024
Version number12