Cellosaurus vdR2-4 (CVCL_JG31)
| Cell line name | vdR2-4 |
|---|---|
| Accession | CVCL_JG31 |
| Resource Identification Initiative | To cite this cell line use: vdR2-4 (RRID:CVCL_JG31) |
| Comments | Characteristics: Both alleles of Blm locus were modified for tetracycline-dependent gene regulation. A 4-hydroxytamoxifen-regulatable Cre recombinase, ERT2-Cre-ERT2 was knocked into the Rosa26 locus for constitutive expression. Genetic integration: Method=Homologous recombination; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2). Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6 x 129S4/SvJae. |
| Hierarchy | Parent: CVCL_C865 (v6.5) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Web pages | Info; EGTC; -; https://web.archive.org/web/20201202094525/http://egtc.jp/action/access/cellline_detail?cellline=vdR2-4+ |
| Cross-references | |
| Cell line collections (Providers) | JCRB; JCRB1658 |
| Encyclopedic resources | Wikidata; Q54992977 |
| Entry history | |
| Entry creation | 15-May-2017 |
| Last entry update | 10-Apr-2025 |
| Version number | 6 |