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Cellosaurus CHO-K1 tsTM18 (CVCL_J507)

[Text version]
Cell line name CHO-K1 tsTM18
Synonyms tsTM18
Accession CVCL_J507
Resource Identification Initiative To cite this cell line use: CHO-K1 tsTM18 (RRID:CVCL_J507)
Comments Characteristics: Temperature sensitive. Exhibits chromosomal instability and cell-cycle arrest at S and G2 phases with decreased DNA synthesis at the non-permissive temperature of 39 Celsius (PubMed=15878348).
Doubling time: 31 hours (PubMed=2237640).
Transformant: ChEBI; CHEBI:21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Mutation; UniProtKB; Q76B40; Smu1; Simple; p.Gly489Arg (c.1465G>A); Zygosity=Homozygous (PubMed=15878348).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=2237640; DOI=10.1007/BF01233196
Tsuji H., Matsudo Y., Tsuji S., Hanaoka F., Hyodo M., Hori T.-a.
Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting chromosomal instability and reduced DNA synthesis at nonpermissive temperature.
Somat. Cell Mol. Genet. 16:461-476(1990)

PubMed=15878348; DOI=10.1016/j.yexcr.2005.02.017
Sugaya K., Hongo E., Tsuji H.
A temperature-sensitive mutation in the WD repeat-containing protein Smu1 is related to maintenance of chromosome integrity.
Exp. Cell Res. 306:242-251(2005)

Cross-references
Encyclopedic resources Wikidata; Q54973163
Entry history
Entry creation11-Feb-2013
Last entry update30-Jan-2024
Version number10