ID   CHO-K1 tsTM18
AC   CVCL_J507
SY   tsTM18
DR   Wikidata; Q54973163
RX   PubMed=2237640;
RX   PubMed=15878348;
CC   Characteristics: Temperature sensitive. Exhibits chromosomal instability and cell-cycle arrest at S and G2 phases with decreased DNA synthesis at the non-permissive temperature of 39 Celsius (PubMed=15878348).
CC   Doubling time: 31 hours (PubMed=2237640).
CC   Sequence variation: Mutation; UniProtKB; Q76B40; Smu1; Simple; p.Gly489Arg (c.1465G>A); Zygosity=Homozygous (PubMed=15878348).
CC   Transformant: ChEBI; CHEBI:21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_0214 ! CHO-K1
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 11-02-13; Last updated: 30-01-24; Version: 10
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RX   PubMed=2237640; DOI=10.1007/BF01233196;
RA   Tsuji H., Matsudo Y., Tsuji S., Hanaoka F., Hyodo M., Hori T.-a.;
RT   "Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting
RT   chromosomal instability and reduced DNA synthesis at nonpermissive
RT   temperature.";
RL   Somat. Cell Mol. Genet. 16:461-476(1990).
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RX   PubMed=15878348; DOI=10.1016/j.yexcr.2005.02.017;
RA   Sugaya K., Hongo E., Tsuji H.;
RT   "A temperature-sensitive mutation in the WD repeat-containing protein
RT   Smu1 is related to maintenance of chromosome integrity.";
RL   Exp. Cell Res. 306:242-251(2005).
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