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Cellosaurus RPMI-8226/Dox6 (CVCL_J432)

[Text version]
Cell line name RPMI-8226/Dox6
Synonyms RPMI 8226/DOX6; 8226/DOX6; 8226/Dox6; 8226Dox6; DOX-6; Dox6
Accession CVCL_J432
Resource Identification Initiative To cite this cell line use: RPMI-8226/Dox6 (RRID:CVCL_J432)
Comments Population: African American.
Characteristics: Resistant to 6 x 10-8 M of doxorubicin (PubMed=2563664).
Characteristics: Produces IgG lambda (from parent cell line).
Selected for resistance to: ChEBI; CHEBI_28748; Doxorubicin (Adriablastin; Adriamycin).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: Plasma cell; CL=CL_0000786.
Sequence variations
  • Gene fusion; HGNC; HGNC:5722; IGKJ4 + HGNC; HGNC:5783; IGKV2-28; Name(s)=IGKV2-28-IGKJ4 (from parent cell line).
  • Gene fusion; HGNC; HGNC:5865; IGLJ3 + HGNC; HGNC:5888; IGLV2-14; Name(s)=IGLV2-14-IGLJ3 (from parent cell line).
  • Mutation; HGNC; HGNC:3236; EGFR; Simple; p.Thr751Ile (c.2252C>T); ClinVar=VCV000177773; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ala (c.35G>C); ClinVar=VCV000045122; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Glu285Lys (c.853G>A); ClinVar=VCV000420133; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:12033; TRAF3; Simple; p.Lys191Leufs*60; Zygosity=Unspecified (from parent cell line).
Disease Plasma cell myeloma (NCIt: C3242)
Multiple myeloma (ORDO: Orphanet_29073)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0014 (RPMI-8226)
Sex of cell Male
Age at sampling 61Y
Category Cancer cell line
Publications

PubMed=2563664; DOI=10.1182/blood.V73.3.747.747
William S. Dalton, Thomas M. Grogan, James A. Rybski, Riekeld J. Scheper, Lynne C. Richter, Jenai Kailey, Henk J. Broxterman, Herbert Michael Pinedo, Sydney Elias Salmon;
Immunohistochemical detection and quantitation of P-glycoprotein in multiple drug-resistant human myeloma cells: association with level of drug resistance and drug accumulation.
Blood 73:747-752(1989)

PubMed=2569359
Alex W. Tong, Jennifer Lee, Ru-Mun Wang, William S. Dalton, Takashi Tsuruo, Joseph Wayne Fay, Marvin Jules Stone;
Elimination of chemoresistant multiple myeloma clonogenic colony-forming cells by combined treatment with a plasma cell-reactive monoclonal antibody and a P-glycoprotein-reactive monoclonal antibody.
Cancer Res. 49:4829-4834(1989)

PubMed=10070958
Lori A. Hazlehurst, Nils E. Foley, Mary C. Gleason-Guzman, Miles P. Hacker, Anne Elizabeth Cress, Lee M. Greenberger, Mariska C. de Jong, William S. Dalton;
Multiple mechanisms confer drug resistance to mitoxantrone in the human 8226 myeloma cell line.
Cancer Res. 59:1021-1028(1999)

Cross-references
Cell line databases/resources cancercelllines; CVCL_J432
Encyclopedic resources Wikidata; Q54951247
Entry history
Entry creation11-Feb-2013
Last entry update14-Aug-2025
Version number21