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Cellosaurus TWNT-1 (CVCL_J364)

[Text version]
Cell line name TWNT-1
Accession CVCL_J364
Resource Identification Initiative To cite this cell line use: TWNT-1 (RRID:CVCL_J364)
Comments Group: Patented cell line.
Registration: International Depositary Authority, Japanese National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology; FERM BP-7843.
Population: Japanese.
Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the TERT gene thus reverting the cells to a non-immortal phenotype.
Characteristics: Transduced with a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatic stellate cell; CL=CL_0000632.
Disease Epithelioid hemangioendothelioma (NCIt: C3800)
Epithelioid hemangioendothelioma (ORDO: Orphanet_157791)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0390 (LI90)
Sex of cell Female
Age at sampling 55Y
Category Telomerase immortalized cell line
STR profile Source(s): JCRB=JCRB1582

Markers:
AmelogeninX
CSF1PO9,12
D5S81811,13
D7S82010,11
D13S31712
D16S5399,10
TH019
TPOX8,11
vWA16,17

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Publications

PubMed=12811248; DOI=10.1097/01.TP.0000064621.50907.A6
Takamasa Watanabe, Norikuni Shibata, Karen A. Westerman, Teru Okitsu, Jean-Etienne Allain, Masakiyo Sakaguchi, Toshinori Totsugawa, Masanobu Maruyama, Toshihisa Matsumura, Hirofumi Noguchi, Shinichiro Yamamoto ...Show all 18 authors... , Masaki Hikida, Akira Ohmori, Michael G. Reth, Anne Weber, Noriaki Tanaka, Philippe Leboulch, Naoya Kobayashi; Show fewer authors
Establishment of immortalized human hepatic stellate scavenger cells to develop bioartificial livers.
Transplantation 75:1873-1880(2003)

Patent=US7521234
Naoya Kobayashi, Philippe Leboulch, Noriaki Tanaka, Toshiyoshi Fujiwara, Toshinori Totsugawa;
Mammalian immortalized liver cell.
Patent number US7521234, 21-Apr-2009

Cross-references
Cell line collections (Providers) JCRB; JCRB1582
Encyclopedic resources Wikidata; Q54973428
Entry history
Entry creation11-Feb-2013
Last entry update10-Apr-2025
Version number21