ID   TWNT-1
AC   CVCL_J364
DR   JCRB; JCRB1582
DR   Wikidata; Q54973428
RX   Patent=US7521234;
RX   PubMed=12811248;
CC   Group: Patented cell line.
CC   Registration: International Depositary Authority, Japanese National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology; FERM BP-7843.
CC   Population: Japanese.
CC   Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the TERT gene thus reverting the cells to a non-immortal phenotype.
CC   Characteristics: Transduced with a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites.
CC   Transfected with: HGNC; 11730; TERT.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatic stellate cell; CL=CL_0000632.
ST   Source(s): JCRB
ST   Amelogenin: X
ST   CSF1PO: 9,12
ST   D13S317: 12
ST   D16S539: 9,10
ST   D5S818: 11,13
ST   D7S820: 10,11
ST   TH01: 9
ST   TPOX: 8,11
ST   vWA: 16,17
DI   NCIt; C3800; Epithelioid hemangioendothelioma
DI   ORDO; Orphanet_157791; Epithelioid hemangioendothelioma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0390 ! LI90
SX   Female
AG   55Y
CA   Telomerase immortalized cell line
DT   Created: 11-02-13; Last updated: 29-06-23; Version: 17
//
RX   Patent=US7521234;
RA   Kobayashi N., Leboulch P., Tanaka N., Fujiwara T., Totsugawa T.;
RT   "Mammalian immortalized liver cell.";
RL   Patent number US7521234, 21-Apr-2009.
//
RX   PubMed=12811248; DOI=10.1097/01.TP.0000064621.50907.A6;
RA   Watanabe T., Shibata N., Westerman K.A., Okitsu T., Allain J.-E.,
RA   Sakaguchi M., Totsugawa T., Maruyama M., Matsumura T., Noguchi H.,
RA   Yamamoto S., Hikida M., Ohmori A., Reth M.G., Weber A., Tanaka N.,
RA   Leboulch P., Kobayashi N.;
RT   "Establishment of immortalized human hepatic stellate scavenger cells
RT   to develop bioartificial livers.";
RL   Transplantation 75:1873-1880(2003).
//