Cellosaurus Nd (CVCL_IU77)
| Cell line name | Nd |
|---|---|
| Synonyms | Nd ES; Nanog dynamics |
| Accession | CVCL_IU77 |
| Resource Identification Initiative | To cite this cell line use: Nd (RRID:CVCL_IU77) |
| Comments | Characteristics: Provides an accurate and measurable read-out for the dynamic levels of Nanog expression. Characteristics: Contains a transgenic bacterial artificial chromosome (BAC) centered around the Nanog gene but where the Nanog coding sequence was replaced with VNP, a short-lived fluorescent reporter consisting of a Venus yellow fluorescent protein fused to a nuclear localization signal (NLS) and to a degradation signal (PEST). VNP expression is under the control of the Nanog regulatory regions. Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: 129P2/Ola. |
| Hierarchy | Parent: CVCL_9108 (ES-E14TG2a) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=23527287; DOI=10.1371/journal.pone.0059928 |
| Cross-references | |
| Cell line collections (Providers) | ECACC; 16010601 |
| Encyclopedic resources | Wikidata; Q54908298 |
| Entry history | |
| Entry creation | 03-Mar-2017 |
| Last entry update | 29-Jun-2023 |
| Version number | 6 |