ID   Nd
AC   CVCL_IU77
SY   Nd ES; Nanog dynamics
DR   ECACC; 16010601
DR   Wikidata; Q54908298
RX   PubMed=23527287;
CC   Characteristics: Provides an accurate and measurable read-out for the dynamic levels of Nanog expression.
CC   Characteristics: Contains a transgenic bacterial artificial chromosome (BAC) centered around the Nanog gene but where the Nanog coding sequence was replaced with VNP, a short-lived fluorescent reporter consisting of a Venus yellow fluorescent protein fused to a nuclear localization signal (NLS) and to a degradation signal (PEST). VNP expression is under the control of the Nanog regulatory regions.
CC   Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
CC   Breed/subspecies: 129P2/Ola.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_9108 ! ES-E14TG2a
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 03-03-17; Last updated: 29-06-23; Version: 6
//
RX   PubMed=23527287; DOI=10.1371/journal.pone.0059928;
RA   Abranches E., Bekman E.P., Henrique D.;
RT   "Generation and characterization of a novel mouse embryonic stem cell
RT   line with a dynamic reporter of Nanog expression.";
RL   PLoS ONE 8:E59928-E59928(2013).
//