• Gene deletion; HGNC; HGNC:11998; TP53; Zygosity=Homozygous (from parent cell line).
  
• Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (from parent cell line).
  
• Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
  
• Mutation; HGNC; HGNC:11989; TOP2A; Simple; p.Arg487Lys (c.1460G>A); ClinVar=VCV000016772; Zygosity=Unspecified; Note=Acquired during resistance selection process (PubMed=1311390).
  

PubMed=2550442; DOI=10.1016/S0021-9258(19)84722-1
Leonard A. Zwelling, Michael Hinds, Diana Chan, Janice Mayes, Kiem Lan Sie, Elizabeth Parker, Lynn Silberman, Ann Radcliffe, Miloslav Beran, Mark Blick;
Characterization of an amsacrine-resistant line of human leukemia cells. Evidence for a drug-resistant form of topoisomerase II.
J. Biol. Chem. 264:16411-16420(1989)

PubMed=1651812
Michael Hinds, Karl Deisseroth, Janice Mayes, Elizabeth Altschuler, Ruud Jansen, Fred D. Ledley, Leonard A. Zwelling;
Identification of a point mutation in the topoisomerase II gene from a human leukemia cell line containing an amsacrine-resistant form of topoisomerase II.
Cancer Res. 51:4729-4731(1991)

PubMed=1311390; DOI=10.1016/0022-2836(92)90245-F
Ming-Sheng Lee, James Chuo Wang, Miloslav Beran;
Two independent amsacrine-resistant human myeloid leukemia cell lines share an identical point mutation in the 170 kDa form of human topoisomerase II.
J. Mol. Biol. 223:837-843(1992)