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Cellosaurus Melligen (CVCL_HA63)

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Cell line name Melligen
Accession CVCL_HA63
Resource Identification Initiative To cite this cell line use: Melligen (RRID:CVCL_HA63)
Comments Group: Patented cell line.
Population: Japanese.
Characteristics: Produces insulin in response to extracellular glucose levels that are equivalent to physiological glucose levels.
Characteristics: When transplanted into diabetic immunoincompetent mice, Melligen cells restore normoglycemia.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:4195; GCK.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:6081; INS.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_HA62 (HUH7-ins)
Sex of cell Male
Age at sampling 57Y
Category Cancer cell line
Web pages Info; -; -; https://www.diabetes.co.uk/forum/threads/potential-type-1-diabetes-treatment-receives-us-patent-approval.102547/
Publications

PubMed=26029722; DOI=10.1038/mtm.2015.11; PMCID=PMC4445011
Janet Lawandi, Chang Tao, Bin-Hai Ren, Paul Williams, Dora Ling, M. Anne Swan, Najah T. Nassif, Fraser R. Torpy, Bronwyn A. O'Brien, Ann Margaret Simpson;
Reversal of diabetes following transplantation of an insulin-secreting human liver cell line: Melligen cells.
Mol. Ther. Methods Clin. Dev. 2:15011-15011(2015)

Patent=US10738285
Ann Margaret Simpson, Chang Tao;
Cells genetically modified to comprise pancreatic islet glucokinase and uses thereof.
Patent number US10738285, 11-Aug-2020

Cross-references
Cell line databases/resources cancercelllines; CVCL_HA63
Encyclopedic resources Wikidata; Q54905184
Entry history
Entry creation26-Sep-2016
Last entry update14-Aug-2025
Version number18