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Cellosaurus HEP G2ins/g (CVCL_HA61)

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Cell line name HEP G2ins/g
Synonyms HEPG2ins/g; HepG2ins/g
Accession CVCL_HA61
Resource Identification Initiative To cite this cell line use: HEP G2ins/g (RRID:CVCL_HA61)
Comments Group: Patented cell line.
Population: Caucasian.
Characteristics: Synthesizes, stores and secretes insulin in a regulated manner when exposed to glucose in tissue culture.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:6081; INS.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11006; SLC2A2.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Hepatoblastoma (NCIt: C3728)
Hepatoblastoma (ORDO: Orphanet_449)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_HA60 (HEP G2ins)
Sex of cell Male
Age at sampling 15Y
Category Cancer cell line
Publications

Patent=WO1995032740A1
Bernard Edward Tuch, Ann Margaret Simpson, Glenn Mark Marshall;
Transformed human hepatoma cell line which releases insulin.
Patent number WO1995032740A1, 07-Dec-1995

PubMed=9425444; DOI=10.1038/sj.gt.3300527
Ann Margaret Simpson, Glenn Mark Marshall, Bernard Edward Tuch, Lori Maxwell, Barbara Szymanska, Jian Tu, Sandy Beynon, M. Anne Swan, Marta Camacho;
Gene therapy of diabetes: glucose-stimulated insulin secretion in a human hepatoma cell line (HEP G2ins/g).
Gene Ther. 4:1202-1215(1997)

PubMed=12958175; DOI=10.1096/fj.02-0051fje; PMCID=PMC2132862
Guo-Jun Liu, Ann Margaret Simpson, M. Anne Swan, Chang Tao, Bernard Edward Tuch, Russell M. Crawford, Aleksandar Jovanovic, Donald K. Martin;
ATP-sensitive potassium channels induced in liver cells after transfection with insulin cDNA and the GLUT 2 transporter regulate glucose-stimulated insulin secretion.
FASEB J. 17:1682-1684(2003)

Cross-references
Cell line databases/resources cancercelllines; CVCL_HA61
Encyclopedic resources Wikidata; Q54882749
Entry history
Entry creation26-Sep-2016
Last entry update14-Aug-2025
Version number21