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Cellosaurus V79-rCYP2B1 (CVCL_GR60)

[Text version]
Cell line name V79-rCYP2B1
Synonyms V79MZr2B1; V79/SD1; SD1; SD-1
Accession CVCL_GR60
Resource Identification Initiative To cite this cell line use: V79-rCYP2B1 (RRID:CVCL_GR60)
Comments Karyotypic information: 21,X,-7,-8der,+mar (PubMed=10474822).
Genetic integration: Method=Transfection/transduction; Gene=RGD; 2466; Cyp2b1.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Cell type: Fibroblast of lung; CL=CL_0002553.
Sequence variations
  • Mutation; UniProtKB; O09185; Tp53; Simple; p.Leu133Gln (c.398T>A); Zygosity=Unspecified (from parent cell line).
  • Mutation; UniProtKB; O09185; Tp53; Simple; p.Cys135Trp (c.405C>G); Zygosity=Unspecified (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_4V36 (V79MZ)
Sex of cell Male
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=3137560; DOI=10.1073/pnas.85.16.5769; PMCID=PMC281846
Doehmer J., Dogra S., Friedberg T., Monier S., Adesnik M., Glatt H.-R., Oesch F.
Stable expression of rat cytochrome P-450IIB1 cDNA in Chinese hamster cells (V79) and metabolic activation of aflatoxin B1.
Proc. Natl. Acad. Sci. U.S.A. 85:5769-5773(1988)

PubMed=1800893; DOI=10.1093/mutage/6.6.461
Ellard S., Mohammed Y., Dogra S., Wolfel C., Doehmer J., Parry J.M.
The use of genetically engineered V79 Chinese hamster cultures expressing rat liver CYP1A1, 1A2 and 2B1 cDNAs in micronucleus assays.
Mutagenesis 6:461-470(1991)

PubMed=1441600; DOI=10.3109/00498259209051863
Doehmer J., Wolfel C., Dogra S., Doehmer C., Seidel A., Platt K.-L., Oesch F., Glatt H.-R.
Applications of stable V79-derived cell lines expressing rat cytochromes P4501A1, 1A2, and 2B1.
Xenobiotica 22:1093-1099(1992)

PubMed=7565906; DOI=10.1016/0165-7992(95)00034-8
Simi S., Morelli S., Gervasi P.G., Rainaldi G.
Clastogenicity of anthraquinones in V79 and in three derived cell lines expressing P450 enzymes.
Mutat. Res. 347:151-156(1995)

PubMed=8596476; DOI=10.1093/mutage/10.6.549
Ellard S., James S.A., Parry E.M., Parry J.M.
A genetically engineered V79 cell line SD1 expressing rat CYP2B1 exhibits chromosomal instability at the integration site of the transfected DNA.
Mutagenesis 10:549-554(1995)

PubMed=10474822; DOI=10.1093/mutage/14.1.57
Simi S., Xiao Y., Campagna M., Doehmer J., Darroudi F.
Dual-colour FISH analysis to characterize a marker chromosome in cytochrome P450 2B1 recombinant V79 Chinese hamster cells.
Mutagenesis 14:57-61(1999)

Cross-references
Cell line databases/resources TOKU-E; 4043
Encyclopedic resources Wikidata; Q54992618
Entry history
Entry creation26-Sep-2016
Last entry update10-Sep-2024
Version number11