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Cellosaurus V79MZr2B1 (CVCL_GR60)

[Text version]
Cell line name V79MZr2B1
Synonyms V79-rCYP2B1; V79/SD1; SD1; SD-1
Accession CVCL_GR60
Resource Identification Initiative To cite this cell line use: V79MZr2B1 (RRID:CVCL_GR60)
Comments Karyotypic information: 21,X,-7,-8der,+mar (PubMed=10474822).
Genetic integration: Method=Transfection; Gene=RGD; 2466; Cyp2b1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Cell type: Fibroblast of lung; CL=CL_0002553.
Sequence variations
  • Mutation; UniProtKB; O09185; Tp53; Simple; p.Leu133Gln (c.398T>A); Zygosity=Unspecified (from parent cell line).
  • Mutation; UniProtKB; O09185; Tp53; Simple; p.Cys135Trp (c.405C>G); Zygosity=Unspecified (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_4V36 (V79MZ)
Sex of cell Male
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=3137560; DOI=10.1073/pnas.85.16.5769; PMCID=PMC281846
Johannes Doehmer, Satish Dogra, Thomas Friedberg, Solange Monier, Milton Adesnik, Hans-Ruedolf Glatt, Franz Oesch;
Stable expression of rat cytochrome P-450IIB1 cDNA in Chinese hamster cells (V79) and metabolic activation of aflatoxin B1.
Proc. Natl. Acad. Sci. U.S.A. 85:5769-5773(1988)

PubMed=1800893; DOI=10.1093/mutage/6.6.461
Sian Ellard, Yaseen Mohammed, Satish Dogra, Catherine Wolfel, Johannes Doehmer, James M. Parry;
The use of genetically engineered V79 Chinese hamster cultures expressing rat liver CYP1A1, 1A2 and 2B1 cDNAs in micronucleus assays.
Mutagenesis 6:461-470(1991)

PubMed=1441600; DOI=10.3109/00498259209051863
Johannes Doehmer, Catherine Wolfel, Satish Dogra, C. Doehmer, Albrecht Seidel, Karl-Ludwig Platt, Franz Oesch, Hans-Ruedolf Glatt;
Applications of stable V79-derived cell lines expressing rat cytochromes P4501A1, 1A2, and 2B1.
Xenobiotica 22:1093-1099(1992)

PubMed=7565906; DOI=10.1016/0165-7992(95)00034-8
Silvana Simi, S. Morelli, Pier Giovanni Gervasi, Giuseppe Rainaldi;
Clastogenicity of anthraquinones in V79 and in three derived cell lines expressing P450 enzymes.
Mutat. Res. 347:151-156(1995)

PubMed=8596476; DOI=10.1093/mutage/10.6.549
Sian Ellard, Sally A. James, Elizabeth M. Parry, James M. Parry;
A genetically engineered V79 cell line SD1 expressing rat CYP2B1 exhibits chromosomal instability at the integration site of the transfected DNA.
Mutagenesis 10:549-554(1995)

PubMed=10335445; DOI=10.1081/dmr-100101928
Johannes Doehmer, Jeroen Titus Maria Buters, Andreas Luch, Volker J. Soballa, William M. Baird, Hilary Gonzalez Morrison, John Joseph Stegeman, Alan J. Townsend, William F. Greenlee, Hans-Ruedolf Glatt, Albrecht Seidel ...Show all 13 authors... , Juergen Jacob, Helmut Greim; Show fewer authors
Molecular studies on the toxifying effects by genetically engineered cytochromes P450.
Drug Metab. Rev. 31:423-435(1999)

PubMed=10474822; DOI=10.1093/mutage/14.1.57
Silvana Simi, Yun Xiao, Marcello Campagna, Johannes Doehmer, Firouz Darroudi;
Dual-colour FISH analysis to characterize a marker chromosome in cytochrome P450 2B1 recombinant V79 Chinese hamster cells.
Mutagenesis 14:57-61(1999)

DOI=10.1080/10406639908020586
Juergen Jacob, Gottfried Raab, Volker J. Soballa, Andreas Luch, Gernot Grimmer, Helmut Greim, Johannes Doehmer, Hilary Gonzalez Morrison, John Joseph Stegeman, Albrecht Seidel;
Species-dependent metabolism of benzo[c]phenanthrene and dibenzo[a, l]pyrene by various CYP450 isoforms.
Polycycl. Aromat. Compd. 16:191-203(2000)

Cross-references
Cell line databases/resources TOKU-E; 4043
Encyclopedic resources Wikidata; Q54992618
Entry history
Entry creation26-Sep-2016
Last entry update14-Aug-2025
Version number12