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Cellosaurus HT-29-M6 (CVCL_G077)

[Text version]
Cell line name HT-29-M6
Synonyms HT-29 M6; HT29-M6; HT29-5M6; 5M6
Accession CVCL_G077
Resource Identification Initiative To cite this cell line use: HT-29-M6 (RRID:CVCL_G077)
Comments Population: Caucasian.
Selected for resistance to: ChEBI; CHEBI:44185; Methotrexate (MTX).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0320 (HT-29)
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line

PubMed=9579576; DOI=10.1002/(SICI)1097-0215(19980504)76:3<383::AID-IJC16>3.0.CO;2-C
Lesuffleur T., Violette S., Vasile-Pandrea I., Dussaulx E., Barbat A., Muleris M., Zweibaum A.
Resistance to high concentrations of methotrexate and 5-fluorouracil of differentiated HT-29 colon-cancer cells is restricted to cells of enterocytic phenotype.
Int. J. Cancer 76:383-392(1998)

PubMed=17311291; DOI=10.1002/jcp.20999
Mayo C., Lloreta J., Real F.X., Mayol X.
In vitro differentiation of HT-29 M6 mucus-secreting colon cancer cells involves a trychostatin A and p27(KIP1)-inducible transcriptional program of gene expression.
J. Cell. Physiol. 212:42-50(2007)

Cell line databases/resources cancercelllines; CVCL_G077
Encyclopedic resources Wikidata; Q54896517
Entry history
Entry creation11-Feb-2013
Last entry update05-Oct-2023
Version number20