Cellosaurus cell line Huh7 GPC3-KO (CVCL

Entry history
Cell line name	Huh7 GPC3-KO
Synonyms	HG3
Accession	CVCL_F6LA
Resource Identification Initiative	To cite this cell line use: Huh7 GPC3-KO (RRID:CVCL_F6LA)
Comments	Population: Japanese. Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:4451; GPC3. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Miscellaneous: Cell line information from personal communication of Fayn, Stanley. Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations	Mutation; HGNC; HGNC:6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line). Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).
Disease	Adult hepatocellular carcinoma (NCIt: C7956) Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0336 (Huh-7)
Sex of cell	Male
Age at sampling	57Y
Category	Cancer cell line
Entry creation	22-Oct-2026
Last entry update	22-Oct-2026
Version number	1