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Cellosaurus HyCyte NCI-H358 stable cell line for hKRAS overexpression (CVCL_F4W3)

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Cell line name HyCyte NCI-H358 stable cell line for hKRAS overexpression
Synonyms NCI-H358 stable cell line for hKRAS overexpression
Accession CVCL_F4W3
Resource Identification Initiative To cite this cell line use: HyCyte NCI-H358 stable cell line for hKRAS overexpression (RRID:CVCL_F4W3)
Comments Population: Caucasian.
Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hKRAS [NM_004985.5]-PGK>puro.
Doubling time: 36-48 hours (Hysigen=CGSC-M26541).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:6407; KRAS.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Cell type: Club cell; CL=CL_0000158.
Sequence variations
  • Gene deletion; HGNC; HGNC:11998; TP53; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Thr75Ala (c.223A>G); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Cys (c.34G>T); ClinVar=VCV000012578; Zygosity=Heterozygous (from parent cell line).
Disease Minimally invasive lung adenocarcinoma (NCIt: C2923)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1559 (NCI-H358)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M26541
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1