ID   HyCyte NCI-H358 stable cell line for hKRAS overexpression
AC   CVCL_F4W3
SY   NCI-H358 stable cell line for hKRAS overexpression
DR   Hysigen; CGSC-M26541
CC   Population: Caucasian.
CC   Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hKRAS [NM_004985.5]-PGK>puro.
CC   Doubling time: 36-48 hours (Hysigen=CGSC-M26541).
CC   Sequence variation: Gene deletion; HGNC; HGNC:11998; TP53; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Thr75Ala (c.223A>G); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Cys (c.34G>T); ClinVar=VCV000012578; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
CC   Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:6407; KRAS.
CC   Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Lung; UBERON=UBERON_0002048.
CC   Cell type: Club cell; CL=CL_0000158.
DI   NCIt; C2923; Minimally invasive lung adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_1559 ! NCI-H358
SX   Male
AG   Age unspecified
CA   Cancer cell line
DT   Created: 25-06-26; Last updated: 25-06-26; Version: 1
//