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Cellosaurus HyCyte MDA-MB-231 stable cell line for hCTSB overexpression (CVCL_F4UJ)

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Cell line name HyCyte MDA-MB-231 stable cell line for hCTSB overexpression
Synonyms MDA-MB-231 stable cell line for hCTSB overexpression
Accession CVCL_F4UJ
Resource Identification Initiative To cite this cell line use: HyCyte MDA-MB-231 stable cell line for hCTSB overexpression (RRID:CVCL_F4UJ)
Comments Population: Caucasian.
Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>3×flag-hCTSB[NM_001908.5]-PGK>Puro.
Doubling time: 24-36 hours (Hysigen=CGSC-M35551).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:2527; CTSB.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Gly464Val (c.1391G>T); ClinVar=VCV000040364; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg280Lys (c.839G>A); ClinVar=VCV000376657; Zygosity=Homozygous (from parent cell line).
Disease Breast adenocarcinoma (NCIt: C5214)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0062 (MDA-MB-231)
Sex of cell Female
Age at sampling 51Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M35551
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1