ID   HyCyte MDA-MB-231 stable cell line for hCTSB overexpression
AC   CVCL_F4UJ
SY   MDA-MB-231 stable cell line for hCTSB overexpression
DR   Hysigen; CGSC-M35551
CC   Population: Caucasian.
CC   Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>3×flag-hCTSB[NM_001908.5]-PGK>Puro.
CC   Doubling time: 24-36 hours (Hysigen=CGSC-M35551).
CC   Sequence variation: Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Gly464Val (c.1391G>T); ClinVar=VCV000040364; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg280Lys (c.839G>A); ClinVar=VCV000376657; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
CC   Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:2527; CTSB.
CC   Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
DI   NCIt; C5214; Breast adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0062 ! MDA-MB-231
SX   Female
AG   51Y
CA   Cancer cell line
DT   Created: 25-06-26; Last updated: 25-06-26; Version: 1
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