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Cellosaurus HyCyte Nthy-ori 3-1 stable cell line for hBRAF overexpression (CVCL_F4TS)

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Cell line name HyCyte Nthy-ori 3-1 stable cell line for hBRAF overexpression
Synonyms Nthy-ori 3-1 stable cell line for hBRAF overexpression
Accession CVCL_F4TS
Resource Identification Initiative To cite this cell line use: HyCyte Nthy-ori 3-1 stable cell line for hBRAF overexpression (RRID:CVCL_F4TS)
Comments Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hBRAF-PGK>Puro.
Doubling time: 24-48 hours (Hysigen=CGSC-M25021).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:1097; BRAF.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Derived from site: In situ; Thyroid gland; UBERON=UBERON_0002046.
Cell type: Follicular cell; CL=CL_0002258.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_2659 (Nthy-ori 3-1)
Sex of cell Female
Age at sampling 35Y
Category Transformed cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M25021
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1