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Cellosaurus HyCyte Huh-7 stable cell line for hBSG overexpression (CVCL_F4SK)

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Cell line name HyCyte Huh-7 stable cell line for hBSG overexpression
Synonyms Huh-7 stable cell line for hBSG overexpression
Accession CVCL_F4SK
Resource Identification Initiative To cite this cell line use: HyCyte Huh-7 stable cell line for hBSG overexpression (RRID:CVCL_F4SK)
Comments Population: Japanese.
Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hBSG[NM_001728.4]-PGK>Puro.
Doubling time: 36-48 hours (Hysigen=CGSC-M33661).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:1116; BSG.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0336 (Huh-7)
Sex of cell Male
Age at sampling 57Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M33661
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1