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Cellosaurus HyCyte MRC-5 stable cell line for hRUNX2 overexpression (CVCL_F4SH)

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Cell line name HyCyte MRC-5 stable cell line for hRUNX2 overexpression
Synonyms MRC-5 stable cell line for hRUNX2 overexpression
Accession CVCL_F4SH
Resource Identification Initiative To cite this cell line use: HyCyte MRC-5 stable cell line for hRUNX2 overexpression (RRID:CVCL_F4SH)
Comments Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hRUNX2[NM_001024630.4]-PGK>Puro.
Doubling time: 48-60 hours (Hysigen=CGSC-M33611).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:10472; RUNX2.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Fetal lung; UBERON=UBERON_0005597.
Cell type: Fibroblast of lung; CL=CL_0002553.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0440 (MRC-5)
Sex of cell Male
Age at sampling 14FW
Category Finite cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M33611
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1