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Cellosaurus HyCyte U2OS stable cell line for hPIEZO1 overexpression (CVCL_F4RT)

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Cell line name HyCyte U2OS stable cell line for hPIEZO1 overexpression
Synonyms U2OS stable cell line for hPIEZO1 overexpression
Accession CVCL_F4RT
Resource Identification Initiative To cite this cell line use: HyCyte U2OS stable cell line for hPIEZO1 overexpression (RRID:CVCL_F4RT)
Comments Population: Caucasian.
Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hPIEZO1[NM_001142864.4]-PGK>Puro.
Doubling time: 18-24 hours (Hysigen=CGSC-M32871).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:28993; PIEZO1.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; None_reported; -; Zygosity=- (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M32871
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1