ID   HyCyte U2OS stable cell line for hPIEZO1 overexpression
AC   CVCL_F4RT
SY   U2OS stable cell line for hPIEZO1 overexpression
DR   Hysigen; CGSC-M32871
CC   Population: Caucasian.
CC   Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hPIEZO1[NM_001142864.4]-PGK>Puro.
CC   Doubling time: 18-24 hours (Hysigen=CGSC-M32871).
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; None_reported; -; Zygosity=- (from parent cell line).
CC   Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
CC   Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:28993; PIEZO1.
CC   Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 25-06-26; Last updated: 25-06-26; Version: 1
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