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Cellosaurus HyCyte PLC/PRF/5 stable cell line for hMFSD2A overexpression (CVCL_F4RP)

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Cell line name HyCyte PLC/PRF/5 stable cell line for hMFSD2A overexpression
Synonyms PLC/PRF/5 stable cell line for hMFSD2A overexpression
Accession CVCL_F4RP
Resource Identification Initiative To cite this cell line use: HyCyte PLC/PRF/5 stable cell line for hMFSD2A overexpression (RRID:CVCL_F4RP)
Comments Population: African; Shangaan.
Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hMFSD2A[NM_001136493.3]-PGK>Puro.
Doubling time: 35-40 hours (Hysigen=CGSC-M32801).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:25897; MFSD2A.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg112Gly (c.334C>G) (p.Pro126Arg, c.377C>G); ClinVar=VCV000233484; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg249Ser (c.747G>T); ClinVar=VCV000012352; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:12363; TSC2; Simple; p.Asn1651Ser (c.4952A>G); ClinVar=VCV000049335; Zygosity=Homozygous (from parent cell line).
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0485 (PLC/PRF/5)
Sex of cell Male
Age at sampling 24Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M32801
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1