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Cellosaurus HyCyte H9c2(2-1) stable cell line for rKeap1 overexpression (CVCL_F4Q8)

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Cell line name HyCyte H9c2(2-1) stable cell line for rKeap1 overexpression
Synonyms H9c2(2-1) stable cell line for rKeap1 overexpression
Accession CVCL_F4Q8
Resource Identification Initiative To cite this cell line use: HyCyte H9c2(2-1) stable cell line for rKeap1 overexpression (RRID:CVCL_F4Q8)
Comments Characteristics: Transfected with pPB[Exp]-EF1A>EYFP-CAG>rKeap1[NM_057152.2]-PGK>puro.
Doubling time: 48-72 hours (Hysigen=CGSC-M30821).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; 8DNLG; EYFP.
Genetic integration: Method=PiggyBac transposition; Gene=RGD; 621619; Keap1.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Embryonic heart, cardiac muscle; UBERON=UBERON_0003084+UBERON_0001133.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: BDIX; RS=RS_0000091.
Hierarchy Parent: CVCL_0286 (H9c2(2-1))
Sex of cell Sex unspecified
Age at sampling Embryo
Category Spontaneously immortalized cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M30821
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1