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Cellosaurus HyCyte NRK-52E stable cell line for rHif1a overexpression (CVCL_F4Q6)

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Cell line name HyCyte NRK-52E stable cell line for rHif1a overexpression
Synonyms NRK-52E stable cell line for rHif1a overexpression
Accession CVCL_F4Q6
Resource Identification Initiative To cite this cell line use: HyCyte NRK-52E stable cell line for rHif1a overexpression (RRID:CVCL_F4Q6)
Comments Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>rHif1a[NM_024359.2]-PGK>Puro.
Doubling time: 24-36 hours (Hysigen=CGSC-M30781).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=RGD; 61928; Hif1a.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Osborne-Mendel; RS=RS_0000629.
Hierarchy Parent: CVCL_0468 (NRK-52E)
Category Spontaneously immortalized cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M30781
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1