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Cellosaurus HyCyte MC3T3-E1 stable cell line for mNp overexpression (CVCL_F4PU)

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Cell line name HyCyte MC3T3-E1 stable cell line for mNp overexpression
Synonyms MC3T3-E1 stable cell line for mNp overexpression
Accession CVCL_F4PU
Resource Identification Initiative To cite this cell line use: HyCyte MC3T3-E1 stable cell line for mNp overexpression (RRID:CVCL_F4PU)
Comments Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>mNpy[NM_023456.3]-PGK>Puro.
Doubling time: 24-48 hours (Hysigen=CGSC-M30301).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=MGI; MGI:97374; Npy.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Bone, calvaria; UBERON=UBERON_0004339.
Cell type: Osteoblast; CL=CL_0000062.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Parent: CVCL_0409 (MC3T3-E1)
Sex of cell Sex unspecified
Age at sampling <1D
Category Spontaneously immortalized cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M30301
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1