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Cellosaurus HyCyte NIH 3T3 stable cell line for mHif1a overexpression (CVCL_F4P7)

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Cell line name HyCyte NIH 3T3 stable cell line for mHif1a overexpression
Synonyms NIH 3T3 stable cell line for mHif1a overexpression
Accession CVCL_F4P7
Resource Identification Initiative To cite this cell line use: HyCyte NIH 3T3 stable cell line for mHif1a overexpression (RRID:CVCL_F4P7)
Comments Characteristics: Transfected with pPB[Exp]-EF1A-EGFP>CAG-mHif1a[NM_010431.3]>PGK-Puro.
Doubling time: 24-36 hours (Hysigen=CGSC-M29571).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=MGI; MGI:106918; Hif1a.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.
Hierarchy Parent: CVCL_0594 (NIH 3T3)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M29571
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1