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Cellosaurus HyCyte EA.hy 926 stable cell line for mPDK1 overexpression (CVCL_F4NG)

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Cell line name HyCyte EA.hy 926 stable cell line for mPDK1 overexpression
Synonyms EA.hy 926 stable cell line for mPDK1 overexpression
Accession CVCL_F4NG
Resource Identification Initiative To cite this cell line use: HyCyte EA.hy 926 stable cell line for mPDK1 overexpression (RRID:CVCL_F4NG)
Comments Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hPDK1[NM_002610.5]>PGK-Puro.
Doubling time: 48-60 hours (Hysigen=CGSC-M28311).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:8809; PDK1.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_3901 (EA.hy 926)
Category Hybrid cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M28311
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1