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Cellosaurus HyCyte AGS stable cell line for hHKDC1[ overexpression (CVCL_F4N9)

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Cell line name HyCyte AGS stable cell line for hHKDC1[ overexpression
Synonyms AGS stable cell line for hHKDC1[ overexpression
Accession CVCL_F4N9
Resource Identification Initiative To cite this cell line use: HyCyte AGS stable cell line for hHKDC1[ overexpression (RRID:CVCL_F4N9)
Comments Population: Caucasian.
Characteristics: Transfected with pPB[Exp]-EF1A>EGFP-CAG>hHKDC1[NM_025130.4]-PGK>Puro.
Doubling time: 24-36 hours (Hysigen=CGSC-M33221).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; EGFP.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:23302; HKDC1.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Stomach; UBERON=UBERON_0000945.
Sequence variations
  • Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Gly34Glu (c.101G>A); ClinVar=VCV000017584; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu453Lys (c.1357G>A); ClinVar=VCV000376470; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Ala (c.1634A>C); ClinVar=VCV000013659; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:667; RHOA; Simple; p.Glu64del (c.192_194del); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; None_reported; -; Zygosity=- (from parent cell line).
Disease Gastric adenocarcinoma (NCIt: C4004)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0139 (AGS)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Hysigen; CGSC-M33221
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1