Cellosaurus cell line HyCyte HL-60 stable cell line for hWT1 overexpression (CVCL

Cross-references
Cell line name	HyCyte HL-60 stable cell line for hWT1 overexpression
Synonyms	HL-60 stable cell line for hWT1 overexpression
Accession	CVCL_F4M2
Resource Identification Initiative	To cite this cell line use: HyCyte HL-60 stable cell line for hWT1 overexpression (RRID:CVCL_F4M2)
Comments	Population: Caucasian. Characteristics: Transduced with pLV[Exp]-Puro-CMV>hWT1[NM_024426.6]. Doubling time: 24-36 hours (Hysigen=CGSC-M27901). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:12796; WT1. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations	Gene deletion; HGNC; HGNC:11998; TP53; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
Disease	Adult acute myeloid leukemia with maturation (NCIt: C7961) Acute myeloblastic leukemia with maturation (ORDO: Orphanet_98834)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0002 (HL-60)
Sex of cell	Female
Age at sampling	36Y
Category	Cancer cell line
Cell line collections (Providers)	Hysigen; CGSC-M27901
Entry history
Entry creation	25-Jun-2026
Last entry update	25-Jun-2026
Version number	1