Cellosaurus cell line HyCyte BT-549 KO-hPDK1 (CVCL

Cross-references
Cell line name	HyCyte BT-549 KO-hPDK1
Synonyms	hPDK1 knockout BT-549 cell line
Accession	CVCL_F4IV
Resource Identification Initiative	To cite this cell line use: HyCyte BT-549 KO-hPDK1 (RRID:CVCL_F4IV)
Comments	Population: Caucasian. Doubling time: 36-48 hours (Hysigen=CGKO-M2168). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:8809; PDK1. Derived from site: In situ; Breast; UBERON=UBERON_0000310.
Sequence variations	Gene fusion; HGNC; HGNC:2092; CLTC + HGNC; HGNC:29559; VMP1; Name(s)=CLTC-VMP1 (from parent cell line). Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Val275fs*1 (c.821delG) (p.W274fs); Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:9884; RB1; Simple; c.265_607del343; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg249Ser (c.747G>C); ClinVar=VCV000406598; Zygosity=Homozygous (from parent cell line).
Disease	Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_1092 (BT-549)
Sex of cell	Female
Age at sampling	72Y
Category	Cancer cell line
Cell line collections (Providers)	Hysigen; CGKO-M2168
Entry history
Entry creation	25-Jun-2026
Last entry update	25-Jun-2026
Version number	1