Cellosaurus cell line HyCyte LS174T KO-hCHST4 (CVCL

Cross-references
Cell line name	HyCyte LS174T KO-hCHST4
Synonyms	hCHST4 knockout LS174T cell line
Accession	CVCL_F4E2
Resource Identification Initiative	To cite this cell line use: HyCyte LS174T KO-hCHST4 (RRID:CVCL_F4E2)
Comments	Population: Caucasian. Doubling time: 24-36 hours (Hysigen=CGKO-M3303). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:1972; CHST4. Derived from site: In situ; Colon; UBERON=UBERON_0001155. Cell type: Epithelial cell of colon; CL=CL_0011108.
Sequence variations	Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45Phe (c.134C>T); ClinVar=VCV000017588; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Unspecified (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; None_reported; -; Zygosity=- (from parent cell line).
Disease	Colon adenocarcinoma (NCIt: C4349)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_1384 (LS174T)
Sex of cell	Female
Age at sampling	58Y
Category	Cancer cell line
Cell line collections (Providers)	Hysigen; CGKO-M3303
Entry history
Entry creation	25-Jun-2026
Last entry update	25-Jun-2026
Version number	1