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Cellosaurus Cas9 RPE-1 VPS13C^mStayGold (CVCL_F4CL)

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Cell line name Cas9 RPE-1 VPS13C^mStayGold
Synonyms RPE-1 VPS13C^mStayGold
Accession CVCL_F4CL
Resource Identification Initiative To cite this cell line use: Cas9 RPE-1 VPS13C^mStayGold (RRID:CVCL_F4CL)
Comments Characteristics: Stably expresses Streptococcus pyogenes Cas9 and overexpresses VPS13C internally tagged with the mStayGold fluorescent protein.
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; 5H7QY; mStayGold.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:23594; VPS13C.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Miscellaneous: Cell line information from personal communication of Jessica, Eden.
Derived from site: In situ; Eye, retina, retinal pigment epithelium; UBERON=UBERON_0001782.
Cell type: Retinal pigment epithelial cell; CL=CL_0002586.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_4388 (hTERT-RPE1)
Sex of cell Female
Age at sampling 1Y
Category Telomerase immortalized cell line
Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1